Can you develop coâculture models that combine epithelial and immune cells?

Absolutely. Coâculture systems are one of our core strengths. We can establish direct or indirect contact coâcultures (using transwell inserts or matrixâembedded cells) to study epithelialâimmune crossâtalk, inflammatory cascades, or the impact of test compounds on the local microenvironment.

Contract Research Solutions for Respiratory Diseases

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All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

Custom Primary Cell and Immune Cell Systems for Respiratory Research

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Advanced drug discovery for respiratory diseases demands experimental models that closely mirror human pathophysiology. At Ace Therapeutics, we specialize in providing customizable primary cell and immune cell systems tailored to respiratory research. By leveraging cells derived from healthy and diseased tissues (human and animal), our in vitro models enable more predictive assessment of candidate efficacy, toxicity, and immunomodulatory effects. Whether you are investigating asthma, COPD, pulmonary fibrosis, or lung infections, our flexible solutions help you bridge the gap between simplistic cell lines and complex in vivo studies.

Our Primary Cell and Immune Cell Systems: Models Tailored to Your Needs

We do not simply supply cells; we design and develop custom cell-based systems to answer your specific research questions. The table below illustrates the breadth of models we can generate, along with their typical applications in respiratory drug development.

Model Type	Cell Types Included	Disease Relevance
Airway Epithelial Systems	Bronchial/tracheal epithelial cells (normal & diseased)	Asthma, COPD, cystic fibrosis
Alveolar Models	Alveolar type I/II epithelial cells	IPF, ARDS, emphysema
Pulmonary Immune Cell Systems	Alveolar macrophages, dendritic cells, T cells, neutrophils	Inflammation, infection, autoimmunity
Co-culture Systems	Epithelial + immune cells; fibroblast + immune cells	Fibrosis, allergy, tumour microenvironment
Disease-specific Isolates	Cells from allergen-challenged or transgenic models	Asthma, fibrosis, bacterial infection

All models can be customized regarding cell source (healthy vs. diseased), donor genetics, and culture format (e.g., monolayers, air‑liquid interface, 3D spheroids). We also offer isolation from specific disease model animals upon request.

Integrated Preclinical Services: From Model Development to Functional Analysis

Our expertise goes beyond model construction. We provide a comprehensive suite of downstream analysis services designed to extract maximum value from your primary cell and immune cell systems. These services are performed by experienced scientists and can be adapted to your study objectives.

Model Development & Customization

Primary cell isolation from human tissues (ethically sourced) or rodent models (naïve or diseased)
Cell purification (magnetic bead sorting, FACS) to achieve >90% purity for rare populations
Co-culture set‑up with defined ratios, direct or indirect contact (transwells), and controlled differentiation conditions
Disease modelling using cytokine stimulation, allergen challenge, or hypoxic exposure to recapitulate pathophysiological features

Immune Cell Function Assays

Cytokine/chemokine quantification
Flow cytometry for immunophenotyping, activation markers (CD69, CD25), intracellular cytokines, and proliferation (CFSE or dye dilution)
Phagocytosis and efferocytosis assays using fluorescently labelled particles or apoptotic cells
T‑cell activation and cytotoxicity (CD8+ T‑cell killing of antigen‑presenting targets)
Neutrophil extracellular trap (NET) formation and oxidative burst measurements

Epithelial & Barrier Function Analyses

Transepithelial electrical resistance (TEER) and paracellular flux (FITC‑dextran)
Ciliary beat frequency and mucin production (ELISA, histology)
Wound healing / scratch assays for epithelial repair
Cell viability and toxicity (ATP, LDH release, MTT)

Molecular & Biochemical Endpoints

Gene expression profiling (qPCR, RNA sequencing)
Protein expression (Western blot, immunohistochemistry)
Signalling pathway activation (phospho‑flow, reporter assays)

All assays can be performed in 96‑well or 384‑well formats to support medium‑throughput screening. We deliver raw data, detailed reports, and expert interpretation to facilitate your decision‑making.

Why Choose Ace Therapeutics for Your Primary Cell and Immune Cell Needs?

Respiratory focus: Our scientists have deep domain knowledge in pulmonary biology and immunology, ensuring that your models are designed with disease relevance in mind.
Custom flexibility: We adapt to your timelines and research goals, offering everything from one‑off isolations to long‑term collaborative partnerships.
Quality and reproducibility: Every batch of cells is rigorously tested for viability, purity, and functional responsiveness. We provide transparent QC data with each delivery.
Integrated solutions: With combined expertise in cell biology and immunology, we can manage your project from model generation through to complex functional readouts, saving you time and resources.

Ready to advance your respiratory drug discovery project?

Contact us today to discuss how our custom primary cell and immune cell systems can be tailored to your specific research needs. Our scientific team is here to provide the support you need—from study design to data interpretation.

Frequently Asked Questions (FAQs)

What types of primary cells can you provide for respiratory research?

We offer a wide range of primary cells from human and common laboratory animals (mouse, rat), including bronchial/alveolar epithelial cells, lung fibroblasts, pulmonary microvascular endothelial cells, and multiple immune cell subsets (macrophages, dendritic cells, T cells, B cells, neutrophils). Both normal and disease‑state cells (e.g., from COPD or IPF patients, or from allergen‑challenged animals) are available.

Can you develop co‑culture models that combine epithelial and immune cells?

Absolutely. Co‑culture systems are one of our core strengths. We can establish direct or indirect contact co‑cultures (using transwell inserts or matrix‑embedded cells) to study epithelial–immune cross‑talk, inflammatory cascades, or the impact of test compounds on the local microenvironment.

What downstream analyses are available for immune cell functional studies?

We offer a full panel of immune function assays, including flow cytometry‑based activation and proliferation, multiplex cytokine profiling, phagocytosis, and cytotoxic T‑cell killing. These assays can be performed on single cells or within co‑culture contexts.

Do you isolate primary cells from specific disease models on demand?

Yes. We can isolate cells from a variety of respiratory disease models (e.g., ovalbumin‑ or house dust mite‑induced asthma, bleomycin‑induced fibrosis, LPS‑induced acute lung injury) according to your protocol. Please discuss your requirements with our team.

How do you ensure the quality and reproducibility of your primary cell systems?

Each preparation is subjected to rigorous quality control: cell viability (trypan blue exclusion), purity (lineage‑specific marker staining by flow cytometry or immunocytochemistry), and functional responsiveness (e.g., cytokine release upon stimulation) are assessed. We also optimize culture conditions to maintain phenotypic stability throughout experiments.