Unlock Insights into IBD Research | Specialized Experimental Model Solutions

Understanding the innate immune mechanisms underlying inflammatory bowel disease (IBD) is critical for the development of next-generation biologics and small-molecule therapeutics. Our Anti-CD40–Induced Colitis Model is a well-established, highly reproducible in vivo system designed to recapitulate acute, cytokine-driven intestinal inflammation relevant to human IBD.

Model Overview

Anti-CD40–induced colitis is an innate immune–dominant model of acute intestinal inflammation, established by systemic administration of an agonistic anti-CD40 antibody in T- and B-cell–deficient RAG-/- mice. Activation of the CD40–CD40L signaling axis triggers a rapid cytokine surge—particularly IL-12, IL-23, IL-1β, TNF-α, and IFN-γ—leading to severe colonic inflammation within days.

Comprehensive Readouts and Endpoints

The poster demonstrates a multidimensional evaluation strategy, including:

• In-life assessments

  Body weight changes and Disease Activity Index (DAI) scoring.

• Colon performance metrics

  Colon weight, colon length, and gross pathology.

• Histological analysis

  H&E staining of proximal and distal colon with detailed pathological scoring.

• Molecular and cytokine profiling

  Cytokine protein and mRNA levels in serum and colon tissue (e.g., TNF-α, IFN-γ, IL-6, IL-12, IL-23, IL-17).

• Immune phenotyping

  Analysis of spleen, mesenteric lymph nodes, and intestinal lamina propria

Applications in IBD Drug Development

This poster illustrates how the Anti-CD40–Induced Colitis Model can be applied to:

• Mechanistic studies of innate immune signaling and cytokine pathways
• In vivo efficacy evaluation of biologics and small molecules
• Rapid compound screening in early discovery and lead optimization
• Proof-of-concept studies for immunomodulatory therapies

Integrated, One-Stop In Vivo IBD Services

At Ace Therapeutics, we provide a fully integrated platform supporting Anti-CD40–induced colitis studies—from model establishment to advanced molecular, immunological, imaging, and pathology analyses—ensuring high-quality, reproducible data aligned with your development goals

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