Explore Immune-Driven IBD Mechanisms with TNBS and Oxazolone-Induced Colitis Models

Our TNBS and Oxazolone-induced colitis case study showcases two of the most widely used immune-relevant models for IBD. Discover how our validated platforms can help you evaluate novel therapies, uncover mechanisms, and generate data with real translational impact.

TNBS-Induced Colitis Model

The 2,4,6-trinitrobenzenesulfonic acid (TNBS) model mimics Crohn's disease-like inflammation, driven primarily by Th1- and Th17-mediated immune responses.

Key Features:

• Clinical Symptoms: Weight loss, loose stool/diarrhea, hematochezia (mice), colon shortening.
• Tissue Damage: Histological examination reveals immune cell infiltration, granuloma formation, and transmural inflammation.
• Immune Response: Characterized by a Th1-Th17-mediated immune response, with high levels of IL-12 and TNF-α.

Oxazolone-Induced Colitis Model

The Oxazolone model closely resembles ulcerative colitis, driven by a Th2-polarized immune response.

Key Features:

• Clinical Symptoms: Colon shortening, diarrhea, goblet cell depletion, and weight loss.
• Tissue Damage: Predominantly superficial inflammation and ulceration composed of a mixed granulocytic and lymphocytic infiltration.
• Immune Response: Characterized by an IL-4- and IL-13-dependent Th2 polarized immune response with increased levels of colonic IL-4, IL-5, IL-13, and transforming growth factor β (TGF-β).

Comprehensive Assessments

Our models are supported by a wide range of in-life and endpoint readouts to ensure robust evaluation:

• In-Life Parameters: Body weight change, Disease Activity Index (DAI)
• Histopathology: H&E staining, crypt analysis, mucosal necrosis, inflammatory infiltration
• Immunological and Molecular Profiling: Cytokine expression, immune cell characterization, biomarker discovery

Why Partner with Ace Therapeutics?

• Validated Models: Proven reproducibility and reliability across multiple studies
• Flexibility: Tailored model design for specific drug targets and therapeutic strategies
• Translational Value: Immune mechanisms closely aligned with human IBD subtypes
• Comprehensive Data Package: From survival, DAI, and weight tracking to deep histological and immunological insights

Download our TNBS and oxazolone-induced colitis model case study today and discover how Ace Therapeutics can help accelerate your preclinical IBD research.

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