Unlock Insights into IBD Research | Specialized Experimental Model Solutions

Our Caco-2/THP-1 Co-culture Model is an advanced in vitro platform designed to recreate the dynamic interplay between intestinal epithelial cells and immune cells during intestinal inflammation. By integrating epithelial barrier function with immune-mediated inflammatory responses, this model provides a physiologically relevant system for investigating inflammatory bowel disease (IBD) pathogenesis and evaluating novel therapeutic strategies.

This downloadable brochure provides a comprehensive overview of how epithelial–immune co-culture systems can be used to study barrier dysfunction, inflammatory signaling, and immunomodulatory mechanisms in preclinical IBD research.

Model Overview

The Caco-2/THP-1 co-culture model combines differentiated Caco-2 intestinal epithelial cells with THP-1-derived macrophage-like cells to mimic key components of the intestinal mucosal environment.

Within the co-culture system, epithelial cells establish a polarized intestinal barrier while immune cells provide inflammatory and cytokine-mediated signaling. Following inflammatory stimulation, the model reproduces important features associated with intestinal inflammation, including barrier disruption, altered permeability, and activation of immune responses.

Key Model Advantages

The brochure highlights several features that make our Caco-2/THP-1 Co-culture Model a valuable tool for preclinical research:

• Integrated Epithelial–Immune Interactions
  Combines intestinal epithelial cells and immune cells within a single experimental system, enabling investigation of bidirectional signaling and inflammatory responses.
• Enhanced Biological Relevance
  Provides a more representative model of intestinal inflammation than conventional epithelial monocultures by incorporating immune-mediated mechanisms.
• Quantitative Assessment of Barrier Function
  Supports detailed analysis of epithelial integrity, permeability changes, and treatment-induced barrier restoration.
• Multiparametric Data Generation
  Enables simultaneous evaluation of barrier function, cytokine secretion, immune activation, and gene expression within a single study.

  Readouts and Assays

Our Caco-2/THP-1 Co-culture Model supports a broad range of analytical endpoints, including:

• Transepithelial Electrical Resistance (TEER) measurements
• Intestinal permeability assessment
• Multiplex cytokine profiling
• Inflammatory biomarker analysis
• Gene expression profiling by qPCR
• Tight junction protein evaluation
• Immune cell activation studies
• Immunostimulatory and immunoinhibitory mechanism assessment
• Cell viability and proliferation assays
• Immunofluorescence imaging and cellular characterization

Discover how our Caco-2/THP-1 Co-culture Model can help you investigate intestinal inflammation, evaluate immunomodulatory therapies, and accelerate the development of next-generation treatments for inflammatory bowel disease.

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