Accelerate Preclinical IBD Research with Intestinal Organoids

Organoid technology has become a cornerstone of inflammatory bowel disease (IBD) research, providing powerful and physiologically relevant insights into disease pathology, therapeutic mechanisms of action, and host–microbiota interactions. Continued advances in organoid-based systems now support the development of sophisticated co-culture models within dynamic, IBD-relevant intestinal microenvironments.

This downloadable case study demonstrates how human small intestinal organoids can be leveraged to model IBD-like inflammation, quantify disease-relevant endpoints, and evaluate therapeutic interventions with high biological relevance.

What This Case Study Shows

In this study, intestinal organoids were exposed to DSS-induced inflammatory conditions, recapitulating key pathological features of IBD. A comprehensive panel of functional, molecular, and structural readouts was applied to assess disease progression and treatment response, including:

• Inflammatory gene expression profiling (IL-6, TNF-α, IL-1β, IL-10) using quantitative RT-PCR
• Epithelial barrier integrity analysis via TEER measurements and tight junction protein (ZO-1) immunofluorescence
• Morphological and structural assessment of organoids during both expansion and differentiation phases
• Functional rescue evaluation, demonstrating partial restoration of barrier function following therapeutic intervention

Why Download This Case Study?

By downloading this material, you will gain insight into:

• How human intestinal organoids can faithfully model IBD-relevant inflammation
• Which endpoints and biomarkers are most informative for preclinical decision-making
• How to integrate TEER, immunofluorescence, cytokine profiling, and gene expression into a single, coherent study design
• The advantages of organoid-based systems compared with conventional 2D cultures and animal models
• How this platform can be extended to drug screening, mechanism-of-action studies, and translational research

Download the Case Study

Discover how our Intestinal Organoids Model can help de-risk your IBD programs, accelerate timelines, and generate data that better predicts outcomes.

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