Unlock Insights into IBD Research | Specialized Experimental Model Solutions

Our Co-culture Model of NCM460 and THP-1 Cells is a flexible system that recreates key epithelial–immune interactions of the human intestine. This downloadable brochure provides a detailed overview of how the model can be applied to study intestinal inflammation, barrier integrity, and drug response, enabling faster and more predictive preclinical decision-making.

Model Overview

This co-culture model combines NCM460 human normal colonic epithelial cells with THP-1–derived macrophages, allowing controlled investigation of inflammatory signaling and epithelial responses. THP-1 cells are differentiated using PMA and co-cultured with NCM460 cells—typically in a Transwell configuration—to maintain apical–basolateral communication while preserving distinct epithelial and immune compartments.

The system effectively mimics host–immune interactions in the gut and supports mechanistic studies of inflammation, immune activation, and epithelial barrier dysfunction relevant to IBD

Key Model Advantages

The brochure highlights several features that make this model particularly valuable for IBD research:

• Physiologically relevant inflammation modeling: Induction of pro-inflammatory pathways, including NF-κB activation and cytokine production (TNF-α, IL-1β, IL-6).
• Intestinal barrier integrity assessment: Suitable for evaluating tight junction function, epithelial permeability, and barrier disruption.
• Flexible experimental design: Configurable for direct contact or Transwell co-culture to study epithelial–immune crosstalk.

Readouts and Assays

The brochure details a comprehensive panel of readouts, including:

• Cell viability and cytotoxicity (e.g., CCK-8)
• Barrier function assessment (TEER measurements, tight junction protein expression)
• Inflammatory response analysis (cytokine quantification such as TNF-α, IL-6, IL-1β)

At Ace Therapeutics, we offer customized co-culture model services, including experimental design, assay execution, and data interpretation—delivering high-quality, reproducible data aligned with your preclinical strategy. Discover how our Co-culture Model of NCM460 and THP-1 Cells can help you model intestinal inflammation, assess epithelial barrier integrity, and accelerate your preclinical IBD research.

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