Unlock Insights into IBD Research | Specialized Experimental Model Solutions

Our T Cell Transfer Colitis Model is a well-established and widely accepted in vivo system that closely mirrors the clinical, histopathological, and immunological features of human IBD, including Crohn's disease and ulcerative colitis. This downloadable brochure provides a comprehensive overview of how the model can be applied to accelerate mechanistic studies and long-term efficacy evaluation in preclinical IBD research.

Model Overview

The T cell transfer model is established by adoptive transfer of naïve CD^4⁺CD45RB^high T cells, depleted of regulatory T cells, into immunodeficient RAG-/- mice. This approach induces a gradual onset of chronic pancolitis and small intestinal inflammation over a 5–8 week period.

Driven by pathogenic Th1 and Th17 immune responses, the model reliably reproduces key aspects of human IBD pathogenesis, making it particularly valuable for studying immune regulation, tolerance breakdown, and chronic inflammation.

Comprehensive Endpoints and Readouts

The brochure details a broad and flexible endpoint strategy, including:

• In-life assessments

  Body weight changes, clinical scoring, stool consistency, and Disease Activity Index (DAI).

• Colon performance metrics

  Colon length and weight measurements.

• Histopathological evaluation

  Detailed H&E and immunohistochemical analysis of colon tissue.

• Immune profiling

  Flow cytometry of lamina propria and mesenteric lymph nodes.

• Cytokine and biomarker analysis

  Luminex, ELISA, fecal biomarkers (LCN2, calprotectin, MPO).

• Gene and protein expression

  qPCR and protein-level analysis to support MoA studies.

Applications in Preclinical IBD Drug Development

By downloading the brochure, you'll learn how the T Cell Transfer Colitis Model supports:

• Mechanistic studies of T cell–mediated intestinal inflammation
• Evaluation of immunomodulatory and anti-inflammatory therapies
• Long-term efficacy and durability studies
• Biomarker discovery and translational strategy development

Discover how our T Cell Transfer Colitis Model can help you investigate adaptive immune mechanisms, assess long-term therapeutic efficacy, and advance your IBD drug development programs.

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