At Ace Therapeutics, our seasoned scientists provide expert preclinical glaucoma research services to support the development of your therapeutic strategies. Leveraging our deep expertise in glaucoma modeling, we develop a genetic mouse model of open-angle glaucoma by expressing of mutated myocilin (MYOC) in transgenic (Tg) mice.
Overview of Tg MYOC Mouse Models
MYOC is the first identified gene linked to primary open-angle glaucoma (POAG). Autosomal dominant MYOC mutations account for 2-4% of POAG cases and most juvenile-onset cases. The disease-associated Tyr437His mutation in human MYOC (Tyr423His in mice) induces similar pathogenic protein properties. Tg mice expressing mutant MYOC under its native promoter develop characteristic POAG features, establishing a robust genetic model for investigating glaucoma pathogenesis and therapeutic interventions.
Fig.1 Degeneration of the optic nerve in transgenic mice. (Zhou Y, et al., 2008)
Explore Our Tg MYOC Mouse Models
Ace Therapeutics has deep experience in developing a variety of animal models by genetic engineering technologies. We construct Tg mouse models expressing mutated MYOC and confirm the insertion of the mutated gene through genotyping via polymerase chain reaction (PCR) and Sanger sequencing.
The Tg mouse model recapitulates the key pathological features of human POAG, including impaired mutant protein expression/secretion, elevated intraocular pressure (IOP), progressive retinal ganglion cell (RGC) loss, and visual functional impairment. Our Tg MYOC Mouse Models offer a robust platform for studying POAG mechanisms and evaluating novel therapeutic candidates, providing valuable insights for the development of novel therapeutics.
Our Capabilities
Ace Therapeutics offers a range of support services for our Tg MYOC mouse model, enabling researchers to conduct in-depth investigations into the role of mutant MYOC in glaucoma.
- IOP measurement
Use tonometry techniques to measure IOP in live mice for real-time monitoring of disease progression and treatment efficacy. - Aqueous humor dynamics analysis
Apply gadolinium magnetic resonance imaging (Gd-MRI) to analyze aqueous humor dynamics. - Visual function assessment
Utilize sinusoids at a range of spatial frequencies to stimulate optokinetic responses in mice to evaluate visual function. - Slit lamp examination
Use slit lamp examination to comprehensively evaluate the anterior segment, including iris, lens and cornea.
Ace Therapeutics understands that every project is unique, and we are here to help you find the appropriate solution. Whether you have specific requirements or just want to explore your options, our team of experts is ready to assist you. Please contact us directly to discuss your needs!
Reference
- Zhou Y, et al. Transgenic mice expressing the Tyr437His mutant of human myocilin protein develop glaucoma. Invest Ophthalmol Vis Sci, 2008, 49(5): 1932-1939.