2D Psoriasis Skin Model Development Services

Psoriasis is a chronic, immune-mediated inflammatory skin disease characterized by keratinocyte hyperproliferation, abnormal epidermal differentiation, and excessive infiltration of immune cells. Preclinical evaluation of novel therapeutics requires robust and reproducible models that mimic the pathological mechanisms of psoriasis. Compared with three-dimensional (3D) reconstructed skin systems and animal models, two-dimensional (2D) psoriasis skin models are more economical and better suited for anti-psoriatic drug screening.

Fig. 1 (a) The 2D engineered skin psoriatic cell model and (b) The co-culture system. (Bocheńska K, et al., 2017)

Ace Therapeutics provides high-quality 2D psoriasis skin model development services, designed to support drug discovery, mechanism-of-action studies, and preclinical evaluation.

Types of 2D Psoriasis Skin Models We Provide

Cytokine-Induced 2D Psoriasis Skin Models

Using normal human epidermal keratinocytes (NHEK) and immortalized human keratinocytes (HaCaT), Ace Therapeutics develops cytokine-induced 2D psoriasis skin models by applying combinations of IL-17, IL-22, TNF-α, and IFN-γ to induce psoriatic phenotypes at the molecular and cellular level. These models reproduce hallmark features of psoriasis, including altered proliferation, differentiation, and cytokine release, providing a reliable platform for the screening of anti-psoriatic candidates.

Co-culture Psoriatic Models

Our company provides co-culture models using keratinocytes (KCs) and fibroblasts (FBs) co-cultures to replicate the immune-epidermal interactions that contribute to psoriatic lesion development, as well as primary human epidermal keratinocytes (PHEK) co-cultured with T cells, which allow precise evaluation of T cell-driven keratinocyte activation and cytokine production.

Evaluation of Anti-Psoriatic Efficacy in 2D Psoriasis Skin Models

Ace Therapeutics provides a comprehensive suite of in vitro assays to evaluate the efficacy of drug candidates in 2D psoriasis skin models.

Morphological and Structural Evaluation of Psoriatic Phenotypes

Using advanced imaging and histological analysis, we assist clients in examining epidermal structure, barrier function, and morphological features of psoriasis in response to anti-psoriatic drugs.

Assessment of Keratinocyte Proliferation and Differentiation

Our company offers services to quantify proliferation markers such as Ki-67 and PCNA, alongside differentiation markers including involucrin, filaggrin, and loricrin, enabling clients to evaluate whether anti-psoriatic drugs restore normal keratinocyte behavior disrupted in psoriasis.

Cytokine and Chemokine Profiling in Psoriasis Models

Using ELISA, qPCR, and multiplex assays, our team helps clients measure psoriasis-related inflammatory mediators, including IL-6, IL-8, and CCL20. Our services support clients in analyzing how anti-psoriatic drug candidates reduce the excessive cytokine release that drives psoriatic inflammation.

Gene Expression Profiling of Psoriasis-Related Pathways

We use high-throughput transcriptomic profiling to identify the effects of drug candidates on psoriasis-related signaling pathways, such as NF-κB, STAT3, and the IL-23/IL-17 axis.

With extensive expertise in dermatological research, Ace Therapeutics has developed robust platforms that enable accurate, cost-efficient evaluation of anti-psoriatic drugs. For more information on 2D psoriasis model development services or to discuss how we can support your research, please do not hesitate to contact us .