Humanized Mouse Psoriasis Model Development Services
Psoriasis is a chronic immune-mediated skin disease characterized by keratinocyte hyperproliferation, epidermal thickening, and persistent inflammation driven by dysregulated cytokine networks. Conventional mouse models provide valuable insights but often fail to capture the complexity of the human immune system. Humanized mouse models are engineered to carry human genes, cells, tissues, or functional immune components. Within dermatology research, humanized psoriasis models enable evaluation of the IL-23/IL-17 axis, TNF-α signaling, and other human pathways.
Ace Therapeutics offers specialized humanized psoriasis model development services, enabling clients to study mechanisms of psoriasis and evaluate the efficacy of anti-psoriatic drugs.
How Do We Develop Humanized Psoriasis Models?
Development of Humanized Mouse Models
At Ace Therapeutics, we develop humanized mice that express human cytokines using gene-targeting strategies, including IL-17 and IL-23. In these models, the murine exons encoding the extracellular domain are replaced with their human counterparts, ensuring expression of human cytokine proteins for psoriasis research. We use ELISA and qPCR to quantify the expression levels of human IL-17A and IL-17F. We also apply immunohistochemistry to profile T cell subsets, such as Th17, Th1, and Treg populations.
IMQ-Induced Psoriasis Phenotypes in Humanized Mouse Models
Our experts induce psoriasis lesions in humanized mice by topical application of imiquimod (IMQ). This stimulation drives strong activation of the IL-23/IL-17 axis, resulting in psoriasis phenotypes, including scaling, erythema, and epidermal thickening. We provide H&E staining of the back skin for analysis of hallmark features of psoriasis, including epidermal hyperplasia, parakeratosis, acanthosis, elongated rete ridges, and dermal immune cell infiltration.
How Do We Evaluate Anti-Psoriatic Efficacy Using Humanized Psoriasis Models?
Our company provides preclinical evaluation of the efficacy of anti-psoriatic drugs using humanized psoriasis models. We assess biologics and small molecules for their ability to reduce lesion severity, normalize cytokine levels, restore epidermal architecture, and improve systemic health indicators such as body weight stability. Additionally, we provide immunophenotyping services to analyze changes in T cell subsets and cytokine expression patterns, such as IL-17A, IL-23, and TNF-α.
Why Choose Ace Therapeutics?
- Specialized in psoriasis models. We focus on developing spontaneous, genetically engineered, xenotransplantation, and humanized psoriasis models according to diverse research needs.
- Comprehensive characterization services. We provide multi-level characterization services, including histopathology, immunophenotyping, and molecular assays.
- Customizable solutions. From psoriasis model development to characterization, we help clients address specific research objectives and accelerate drug discovery pipelines.
Ace Therapeutics provides integrated research services using humanized psoriasis models, enabling mechanistic studies, biomarker discovery, and comprehensive preclinical efficacy evaluation to support the development of psoriasis therapeutics. For more information on humanized psoriasis model development services or to discuss how we can support your research, please do not hesitate to contact us.