TH17-Driven Ex Vivo Human Psoriasis Model Development Services

T helper 17 (Th17) cells, characterized by their production of cytokines such as IL-17A, IL-17F, and IL-22, are integral to the inflammatory processes observed in psoriasis. These cells promote keratinocyte proliferation and produce pro-inflammatory mediators, exacerbating skin lesions. Ace Therapeutics provides innovative TH17-driven ex vivo human psoriasis models to support clients' scientific research and anti-psoriasis drug development.

Introduction to TH17-Driven Ex Vivo Human Psoriasis Models

Unlike conventional skin models, TH17-driven ex vivo human psoriasis models retain full skin barrier function, physiological immune cell diversity, and cellular crosstalk. By replicating the IL-23/IL-17 immune axis and cellular crosstalk between Th17 cells and keratinocytes, this model serves as an important tool for evaluating topical and systemic anti-psoriatic therapeutics.

Fig. 1 Immune cells and Th17-associated cytokines implicated in psoriasis pathogenesis. (Eberle FC, et al., 2016)

TH17-Driven Ex Vivo Human Psoriasis Model Development and Characterization Services

Ace Therapeutics provides comprehensive end-to-end development and characterization of customized TH17-driven ex vivo human psoriasis models to meet specific needs in psoriasis research.

Model Development

To develop ex vivo psoriasis models, we use full-thickness human skin biopsies with subcutaneous fat removed and inject an activation cocktail to stimulate skin-resident T cells, mimicking the physiological conditions for studying T-cell responses. We then culture these biopsies in serum-free medium supplemented with key cytokines to sustain the TH17 phenotypes.

Model Characterization

• Cytokine profiling services. Using enzyme-linked immunosorbent assay (ELISA) techniques, we provide cytokine profiling of critical inflammatory markers, such as IL-17A/F, IL-22, IFN-γ, and TNF-α. Our clients can gain insights into the inflammatory status and immune response within the psoriatic skin model.
• Histopathological analysis services. We support clients in assessing epidermal integrity and overall architecture of skin tissues using comprehensive histopathological analyses to understand structural changes associated with psoriasis.
• Biomarker quantification services. We offer biomarker quantification services using immunofluorescence techniques to detect targets such as S100 calcium-binding protein A7 (S100A7), Keratin-16, Ki67, and Caspase-3. These biomarkers reflect keratinocyte activation, proliferation, and apoptosis, offering insights into pathological processes relevant to psoriasis research.
• Gene expression analysis services. We offer quantitative reverse transcription PCR (qRT-PCR) services to analyze the expression levels of various inflammatory markers, unveiling the inflammatory mechanisms underlying psoriasis.

Applications of TH17-Driven Ex Vivo Human Psoriasis Models

Psoriasis Pathogenesis Studies

We provide services to investigate the roles of TH17-mediated immune responses in psoriasis pathogenesis using established models. Our scientists aid in exploring how TH17 cells interact with other immune cells and keratinocytes to shed light on the complex inflammatory networks involved in psoriasis.

Anti-Psoriatic Drug Discovery and Development

Ace Therapeutics employs TH17-driven ex vivo human psoriasis models to facilitate the screening of potential anti-psoriasis drugs. Our scientists also use these models to offer drug efficacy evaluation services, specifically assessing the modulation of TH17 responses and improvement of psoriasis-like symptoms. We assist clients in assessing potential anti-psoriasis therapeutics when administered at the onset of in situ activation (prophylactic setting) or after the establishment of inflammation (therapeutic setting).

Ace Therapeutics offers ex vivo psoriasis model development and characterization services to accelerate the development of anti-psoriasis drugs. For more information on our TH17-driven ex vivo human psoriasis model development services or to discuss how we can support your research, please do not hesitate to contact us.