Psoriasis Modeling Protocol: Mannan-Induced Skin Inflammation in Mice
This protocol provides a practical approach for inducing psoriasiform skin inflammation in mice using mannan, a potent activator of innate immune pathways. Designed for researchers investigating psoriasis mechanisms, the protocol outlines essential procedures, materials, and readouts needed to reliably generate acute skin inflammation that mimics key features of human psoriatic lesions.
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This flyer by Ace Therapeutics introduces a streamlined protocol for developing mannan-induced psoriasiform skin inflammation in mice, supporting preclinical psoriasis research.
What This Protocol Covers
- Experimental setup: Guidance on materials, mouse preparation, and mannan formulation.
- Induction procedure: Step-by-step instructions for applying mannan to initiate acute skin inflammation.
- Sample collection: Suggestions for obtaining tissues suitable for histology, cytokine analysis, and gene expression profiling.
- Troubleshooting notes: Practical tips to enhance reproducibility and minimize variability across experiments.
Advantages of This Protocol
- Rapid onset of mannan-induced psoriasiform inflammation
- Simple, low-complexity procedure requiring no specialized equipment
- Consistent and reproducible induction across experiments
- Well-suited for studying psoriasis mechanisms and evaluating antipsoriatic drugs
