logo

Developing a Ministroke Model in Mouse Barrel Cortex

The protocol present a ministroke model targeting the mouse barrel cortex to study minor stroke and recurrence. Permanent MCA ligation with brief bilateral CCA occlusion produces small, consistent infarcts and low mortality. Lesions in the barrel cortex allow functional evaluation using the vibrissae-evoked forelimb placing test.

Enhance Your Stroke Research With Our Mouse Ministroke Model Protocol

This protocol offers a step-by-step guide for inducing controlled, localized ischemia with minimal mortality and high consistency, providing a reliable platform for:

  • Investigating the molecular and cellular mechanisms of ministroke
  • Evaluating long-term neuroinflammatory and neuroimmune responses
  • Testing novel neuroprotective agents and rehabilitation therapies
  • Exploring stroke recurrence and chronic recovery pathways

Download Now to Access:

  • Detailed surgical workflow
  • Troubleshooting tips
  • Imaging validation (TTC staining)
  • Behavioral test recommendations

Whether your focus is stroke pathology, drug screening, or neuroplasticity, this protocol provides the clarity and reproducibility essential for impactful discoveries.