Transwell Models

Transwell models are widely used to screen new CNS drug candidates to study BBB permeability and explain how the BBB is regulated. As a supplier of high-quality research tools in the field of stroke, Ace Therapeutics scientists are committed to providing researchers with stable and reliable Transwell models products to promote research and development in the field of neuroscience. In addition, we offer a variety of inserts as well as cell lines for constructing Transwell models to support your research needs.

Our Transwell models product is an experimental model used to study blood-brain barrier permeability and understand blood-brain barrier regulatory mechanisms. These models are based on the Transwell cell culture system and simulate the physiological and structural properties of the blood-brain barrier.

Transwell Models Introduction

Transwell models usually consist of two connected indoor culture chambers separated by a layer of cells or cell culture scaffolds. The upper chamber simulates the lumen of cerebral blood vessels, and the lower chamber simulates brain tissue. The porous membrane allows for the diffusion of nutrients, small molecules, and drugs between the upper and lower chambers, mimicking the transport processes that occur across the blood-brain barrier in vivo. Researchers can add blood-brain barrier cell types, such as brain capillary endothelial cells (BMECs), to the upper chamber to mimic the construction of the blood-brain barrier.

Fig. 1. Schematic representation of the Transwell models. (Stone NL, et al., 2019)

You can use Transwell models to investigate the mechanisms that regulate blood-brain barrier permeability, such as tight junction proteins, efflux transporters, and endocytosis pathways, and to understand how different factors, such as diseases or drug treatments, affect BBB function via manipulating these mechanisms.

Reference

Stone NL, England TJ, O'Sullivan SE. A Novel Transwell Blood Brain Barrier Model Using Primary Human Cells. Front Cell Neurosci. 2019, 13:230.

Cat.#	Name	Description	Price
BBM-M-001	Human Blood-Brain Barrier Model	Human Blood-Brain Barrier Model from Ace Therapeutics is developed by culturing brain endothelial cells with pericytes and astrocytes layered in an insert. The model mimics transport properties of the Blood Brain Barrier (BBB) due to the formation of tight junctions, higher expression of specific carriers, or great cell viability. Compared with the monoculture model, our 3D in vivo model of the BBB can truly mimic the BBB by enhancing the polarization of endothelial cells, facilitating the formation of tight junctions, promoting essential endothelial cell-to-cell contact for barrier development, and preventing the dilution of secreted neurotrophic factors.	inquiry
BBM-M-002	Human Blood-Brain Barrier Modeling Kit	Human Blood-Brain Barrier Modeling Kit from Ace Therapeutics co-cultures endothelial cells, pericytes, and astrocytes in a collagen I matrix with a defined serum-free medium to replicate intercellular interactions at the BBB. Our Models come with media, and you can also order additional media. We provide a series of 3D BBB model culture substrates, such as collagens and hydrogels, for you.	inquiry
BBM-M-003	3D Human Blood-Brain Barrier Modeling Kit with HBMEC	3D Human Blood-Brain Barrier Modeling Kit with HBMEC from Ace Therapeutics is developed by culturing human brain microvascular endothelial cells (HBMEC), brain vascular pericytes, and astrocytes at a 1:1:1 ratio. Our Models come with media, and you can also order additional media. We provide a series of 3D BBB model culture substrates, such as collagens and hydrogels, for you.	inquiry
BBM-M-004	Caco-2 Blood Brain Barrier Modeling Kit	Caco-2 Blood Brain Barrier Modeling Kit from Ace Therapeutics is developed by seeding with 21 days of differentiated and polarized Caco-2 cells on the Transwell insert plates. Our Models come with media, and you can also order additional media. We provide a series of 3D organoid BBB model culture substrates, such as collagens and hydrogels, for you.	inquiry
BBM-M-005	MDCKII Blood Brain Barrier Modeling Kit	MDCKII Blood Brain Barrier Modeling Kit from Ace Therapeutics is developed by seeded with 21 days differentiated and polarized Caco-2 and HT-29 cells with an absorptive and mucus-secreting phenotype, respectively on transwell insert plates. Our Models come with media, and you can also order additional media. We provide a series of 3D organoid BBB model culture substrates, such as collagens and hydrogels, for you.	inquiry
BBM-M-006	Ready-to-Use Blood-Brain Barrier Modeling Kit	Ready-to-Use Blood-Brain Barrier Modeling Kit from Ace Therapeutics is developed by co-culturing three cell types, primary cultures of rat (Wistar rat) brain capillary endothelial cells, brain pericytes, and astrocytes, which is an in vitro reconstituted BBB model and shows many of the in vivo BBB features. Our Models come with media, and you can also order additional media. We provide a series of 3D organoid BBB model culture substrates, such as collagens and hydrogels, for you.	inquiry

All of our products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.