Immortalized Cells

The growth and metabolism of primary cells are in a dynamic balance of proliferation, differentiation, senescence and apoptosis. After experience limited culture, primary cells stop proliferating. A normal human primary cell can only replicate and divide 40 to 60 times before it is unable to divide and will age and die due to programmed cell death or apoptosis. Immortalized cells are derived from primary cells that have escaped normal aging and death and have been cultured for a long time. Cell immortalization strategies often use transgenic transfection technology to artificially introduce exogenous immortalization genes EBV, HPV E6/7 and SV40 T into target cells, or to knock out tumor suppressor genes, or to induce immortalization-related gene mutations to establish stable immortalized cell lines. Compared with normal cells that have a certain life cycle and eventually undergo aging and death, immortalized cells can survive for a long time and maintain a relatively stable physiological state, thereby providing researchers with more cell resources and serving as a good in vitro cell models contribute to the development of new treatment strategies and drugs.

Immortalized Cells for BBB Modeling

The blood-brain barrier (BBB) is a highly selective barrier that separates circulating blood from extracellular fluid in the brain. It plays a key role in maintaining brain homeostasis by regulating the movement of various substances in and out of the brain. As a highly stable and reproducible cell line, immortalized cells, including immortalized brain endothelial cells, immortalized astrocytes, and immortalized pericytes, have been widely used for the establishment of BBB models. Researchers can study the function and properties of the BBB and the ability of drugs to penetrate this barrier by using these cell lines. In addition, by using these immortalized cell lines, researchers can study changes in the BBB in various neurological diseases and find new treatment strategies. Overall, the immortalized cell BBB model provides a good platform for researchers to better understand and explore the interaction between the brain and the circulatory system, hleping support progress in the fields of neuroscience and drug development.

A commonly used immortalized cell line for BBB modeling is the hCMEC/D3 cell line, which are derived from human brain microvascular endothelial cells and exhibit many characteristics of the BBB, including high transendothelial resistance and expression of various tight junction proteins such as claudin-5 and occludin. Additionally, hCMEC/D3 cells retain the ability to actively transport molecules across the BBB, mimicking the in vivo function of the barrier...

Ace Therapeutics provides a variety of immortalized cells from humans and animals that can be used individually or in combination to create in vitro models of the BBB that mimic its physiological properties and functions. Our immortalized cells for BBB modeling are immortalize and cultured according to standardized procedures to ensure stable cell quality between different batches.

Additionally, we offer a variety of supplement media designed to balance and replenish specific cell types thus maximizing their life expectancy.