Antifades & Mounting Media

The Antifade fluorescence mounting media range from Ace Therapeutics is a product that improves the quality of images, ensuring clear visibility and preventing them from fading. This product allows for long-term storage and analysis of samples labeled with fluorescent dyes while minimizing any loss in signal intensity. Our antifade fluorescence mounting media is user-friendly, with a refractive index that aligns with perfect optical conditions. Additionally, it effectively prevents photobleaching, preserving the fluorescence of samples during microscopy or imaging experiments.

Here, Ace Therapeutics provides a simple usage protocol for your reference, for more information please contact our scientists.

Available Protocol for use of mounting media

• ICC

1. Drip a small amount of mounting medium on a standard microscope slide.

2. Rinse the coverslip with dH2O and carefully place it on top of the mounting medium, avoiding bubbles.

3. Cover the slides in foil to prevent light exposure and let the mounting medium solidify overnight at 4°C. Alternatively, for faster imaging, the slides can be incubated at room temperature or 37°C for approximately one hour to accelerate the curing process.

• IHC(IF)

1. Mount sections onto subbed or charged microscope slides and let them air dry in the dark until excess liquid evaporates, ensuring the sections are still slightly moist.

2. Apply a small amount of mounting media around the sections (around 50µl, but need to adjust based on section number and thickness), and slowly place a coverslip over the sections, taking care not to introduce any bubbles.

3. To protect from light exposure, wrap the slides in foil and allow the media to cure overnight at a temperature of 4°C before imaging. Alternatively, for faster imaging, the slides can be incubated at room temperature or 37°C for approximately 1 hour to accelerate the curing process.

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