Cat.#: ASSD-102
| Description | DAPI is a blue fluorescent DNA stain that is cell-permeable at high concentrations. This product is commonly used as a nuclear and chromosomal counterstain. It is used preferentially for staining dead cells. DAPI is less effective as a live cell stain because it cannot efficiently cross the cell membrane of living cells. Therefore, higher concentrations may need to be used. Due to DAPI emitting blue, there is very little fluorescence overlap between yellow fluorescence, green fluorescent molecules (such as fluorescein and GFP), or red fluorescent stains (such as Texas Red). It is therefore suitable for multiplex assays. DAPI Staining Solution (1mg/mL) also available. |
| Size | 10 mg, 50 mg |
| Applications | The product is commonly used for cell imaging, cell counting, cell sorting (based on DNA content), apoptosis analysis, and HCA (high content analysis). |
| Chemical Name | 4',6-Diamidino-2-phenylindole dihydrochloride |
| Molecule Weight | 350.24 |
| Molecular formula | C16H15N5.2HCl |
| CAS Number | 28718-90-3 |
| PubChem identifier | 160166 |
| MDL Number | MFCD00012681 |
| Purity | >98% |
| Solubility | Soluble in water (10mg/ml, gentle warming), and in methanol |
| Excitation | 340/360 nm (for ds-DNA) |
| Emission | 488 460 nm (for ds-DNA) |
| Storage | Store at -20° C |
| Shipping | Shipped at 4°C |
| Important | When using this product, cells must be permeabilized and/or fixed so that DAPI can enter the cells and bind to the DNA. |
| Cat.# | Name | Size | Price |
|---|---|---|---|
| ASSD-210 | 7-Amino-4-methyl-3-coumarinacetic acid N-succinimidyl ester (AC210) | 5 mg | inquiry |
| ASSD-126 | Fluorescent H2 antagonist [aminopotentidine] (AC126) | 50 μg | inquiry |
| ASSD-145 | Oxazole Yellow Iodide 1mM solution (in 1mL DMSO) (AC145) | 1 mL | inquiry |
| ASSD-149 | D-Luciferin sodium salt (AC149) | 10 mg, 50 mg, 100 mg | inquiry |
| ASSD-156 | Crystal Violet (AC156) | 1g, 5g | inquiry |
| ASSD-103 | DAPI Staining Solution (1mg/mL) (AC103) | 1 mL, 5 mL, | inquiry |
| ASSD-115 | 7-Amino-4-methylcoumarin (AC115) | 100 mg, 500 mg, 1 g | inquiry |
| ASSD-111 | Propidium Iodide (AC111) | 10 mg, 50 mg | inquiry |
| ASSD-225 | Antifade Fluorescence Mounting Medium with Propidium Iodide (aqueous) (AC225) | 10 mL | inquiry |
| ASSD-218 | 6-Carboxy-2′,7′-dichlorofluorescein-3′,6′-diacetatesuccinimidyl Ester (AC218) | 25 mg | inquiry |
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