Rat Astrocytes (RA) from Ace Therapeutics are isolated from postnatal day 2 rat brains and are cryopreserved as primary cultures. Each vial contains at least 0.5×10^6 cells per ml and is delivered frozen.

Rat Astrocytes-adult (RA-a) from Ace Therapeutics are isolated from adult CD® IGS rat brains and are cryopreserved after purification. Each vial contains at least 0.5×10^6 cells per ml and is delivered frozen.

Porcine Brain Microvascular Endothelial Cells from Ace Therapeutics are isolated from healthy porcine brain tissue. Porcine Brain Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with a gelatin-based solution for 0.5 hours and incubated in Ace Therapeutics’s Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.

Human brain microvascular pericytes (HBMVPCs) perform numerous tasks which include playing a vital part in constructing and maintaining the blood-brain barrier, controlling angiogenesis, ensuring endothelial cell survival, and engulfing cellular waste. Human Brain Microvascular Pericytes from Ace Therapeutics are isolated from normal human brain cortical tissue. Each vial contains at least 5×10^5 cells per ml and is delivered frozen.

Human Astrocytes from Ace Therapeutics are isolated from the human cerebral cortex, cryopreserved at passage one. Each vial contains at least 5×10^5 cells per ml and is delivered frozen.

Rat astrocytes-midbrain (RA-mb) from Ace Therapeutics are isolated from CD® IGS rat midbrain and are cryopreserved at P0. RA-mb are characterized by immunofluorescence with antibodies specific to GFAP. Each vial contains at least 0.5×10^6 cells per ml and is delivered frozen.

Mouse Astrocytes-cerebellar from CD1 from Ace Therapeutics are isolated from a pathogen-free mouse cerebellar and are cryopreserved as primary cultures. Each vial contains at least 0.5×10^6 cells per ml and is delivered frozen.

Characterization: 1. Cell Activity; – Recovery Viability ≥ 80%. 2. Cell Purity; – GFAP-positive cells ≥ 70%. – β-tubulin III- or doublecortin- (Dcx-) positive cells ≤ 10%. – GalC-positive cells ≤ 10%.

Rat Primary Brain Microvascular Endothelial Cells (RPBECs) from Ace Therapeutics are isolated from the brain tissue of 1-day-old neonatal Sprague-Dawley rats. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.

Bovine astrocytes from Ace Therapeutics are isolated from the bovine brain and are cryopreserved at passage one. Bovine astrocytes are characterized by immunofluorescence with antibody specific to GFAP. Each vial contains at least 0.5×10^6 cells per ml and is delivered frozen.