Development of Exosome-Based Stroke Therapy

As one of the main members of extracellular vesicles, exosomes play an important role in regulating intercellular communication, which are released mainly by mesenchymal stromal cells (MSCs). Studies have shown that exosomes released from MSCs can promote the recovery of the damaged brain. Exosomes may promote functional recovery after cerebral ischemia by promoting neurogenesis and endogenous angiogenesis. Exosomes can also act as intercellular signals to neuroregulate cerebrovascular integrity. In addition, exosomes can alleviate neuroinflammatory response after brain injury to promote recovery of the injured brain. Given the diverse activities of exosomes in the damaged brain, Ace Therapeutics provides comprehensive services to develop novel stroke therapies targeting exosomes to advance the development of stroke therapies.

Exosome Isolation Services

For exosomes from different sources, Ace Therapeutics provides scientific methods for exosome isolation to ensure the development of stroke therapy targeting exosomes. Different isolation methods have different advantages and disadvantages. Our scientists will choose the most appropriate isolation method for you according to your exosomes and your experimental protocol.

The methods included differential centrifugation coupled with ultracentrifugation (UC), using anti-EpCAM (epithelial cell adhesion molecule) coated magnetic beads immunoaffinity pull-down, density gradient separation, sequential centrifugal ultrafiltration by tangential flow filtration, and the use of ExoQuick-TC, rapid isolation of exosome by alternating current electrokinetic microarray chip device and using a commercially available size exclusion chromatography column (SEC) for rapid vesicle purification.

Preclinical Evaluation of Drug Candidates for the Treatment of Stroke

As a cell-free exosome therapy, it has many obvious advantages, such as low immunogenicity, no vascular obstructive effect, and reduced secondary microvascular thrombosis, systemic injection of exosomes can cross the blood brain barrier into the brain parenchyma, and can easily modify exosomes to microRNA, and other advantages. Therefore, the pharmacological activities of suitable exosome drug candidates that have been prepared should be further evaluated for further development. Ace Therapeutics provides comprehensive services for promoting exosome-based stroke therapy.

• We have developed both in vitro and in vivo stroke models to evaluate the role of exosomes in the pathological process of stroke.
• The protein types in exosomes are identified by proteomics and the interaction between proteins in exosomes and nerve cells is explored by computer virtual screening and surface plasmon resonance technology.
• The inflammatory response of exosomes to stroke in vitro and in vivo is measured by detecting different proinflammatory factors and chemokines.
• In view of the activity of exosomes in neurogenesis, neural function recovery, neuronal cell proliferation and migration, and others, we provide various detection services.

We offer custom exosome-based stroke therapy development services for the treatment of stroke based on your experimental needs. If you would like to learn more about our services, please feel free to contact us.

1. Lee, E. C., et al., Utility of exosomes in ischemic and hemorrhagic stroke diagnosis and treatment. Int J Mol Sci, 2022. 23(15).