Analysis of Notch Signaling and Neuronal Death in Stroke

The Notch signaling pathway is a key regulator of many fundamental cellular processes. There is growing evidence that Notch signaling is involved in inflammation and oxidative stress, and thus in the progression of cerebrovascular disease. Furthermore, Notch signaling in cerebrovascular disease is associated with apoptosis, angiogenesis, and blood-brain barrier function. In a mouse model of stroke, activation of the Notch signaling pathway exacerbates ischemic brain injury, whereas inhibition of the Notch signaling pathway reduces infarct size and improves functional outcome. The most important component of the Notch signaling pathway is Notch1, which plays a key role in the stroke process. Modulation of Notch signaling may provide new strategies for the treatment of stroke.

Fig. 1. Modulation of Notch signaling may provide new strategies for the treatment of stroke. Fig. 1. Potential association between apoptosis and Notch signaling following stroke. (Cai et al., 2016)

Our Services

Ace Therapeutics provides comprehensive services to analyze the role of Notch signaling and apoptosis in stroke. Our team consists of experts from different fields, including neuroscience, molecular biology, genetics, and bioinformatics. Integrating multidisciplinary approaches, we help clients obtain comprehensive data on Notch signaling in neuronal death during stroke and develop potential strategies for treating ischemic stroke.

Identification and Validation of Notch Signaling Pathway Related Targets for Stroke

We employ bioinformatics and computational biology approaches to analyze large-scale genomic and transcriptomic datasets, allowing us to identify potential signaling networks and key regulators associated with Notch signaling in stroke. In addition, we can analyze the potential association between apoptosis and Notch signaling after stroke by detecting multiple biomarkers of apoptosis.

Targets associated with the Notch signaling pathway include:

Bcl-2 Bax HIF-1α Pin1
Caspase-9 and -3 JNK/p38 ERK miR-100
NF-κB EGFR Jagged2, angiopoietin 1, eNOS Caspase 8
P21/cyclin D PI3K/Akt ROS GSIs

Analysis of Notch Signaling Pathway in Experimental Models of Stroke

Our researchers have extensive experience with Notch1 antisense transgenic mice, animal models of stroke, and in vitro models of stroke using primary neuronal cultures and established cell lines. We use a variety of techniques to study the Notch signaling pathway in stroke.

  • We offer advanced gene expression profiling technologies, such as RNA sequencing and quantitative PCR, to assess the expression levels of various Notch pathway components.
  • By manipulating Notch signaling activity using genetic approaches or pharmacological inhibitors, we can assess its effects on neuronal viability, apoptosis, and inflammation.
  • Our imaging techniques can visualize and quantify neuronal death and assess the efficacy of potential therapeutic interventions targeting the Notch signaling pathway.

With our expertise, state-of-the-art facilities, and collaborative approach, Ace Therapeutics is committed to help clients analyze Notch signaling in the pathogenesis of stroke and develop stroke drugs targeting Notch signaling. If you are interested in our services, please do not hesitate to contact us!

  1. Cai, Z., et al. (2016). Notch signaling in cerebrovascular diseases. Molecular medicine reports, 14(4), 2883-2898.
All of our services are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
Inquiry Basket