Analysis of Ferroptosis Pathways in Stroke

The neurovascular unit (NVU) plays a crucial role in the onset and progression of ischemic stroke and in vascular and neural remodeling after stroke. Ferroptosis is a new form of cell death in NVUs of ischemic stroke. Unlike other types of cell death such as apoptosis, necrosis, autophagy, and pyroptosis, ferroptosis is a regulated form of cell death characterized by massive iron accumulation and iron-dependent lipid peroxidation. An increasing number of studies have shown that pathological changes in ischemic stroke are closely related to ferroptosis, such as iron metabolism disorders, lipid peroxidation, and increased ROS. Therefore, a deeper understanding of the relationship between ferroptosis and stroke may lead to more effective therapeutic strategies.

Fig. 1. A deeper understanding of the relationship between ferroptosis and stroke lead to more effective therapeutic strategies. Fig. 1. Possible molecular mechanisms of ferroptosis and potential therapeutic targets in ischemic stroke. (Wei et al., 2022)

Our Services

Ace Therapeutics provides reliable services to analyze the mechanism of ferroptosis pathways and develop stroke treatment strategies that target ferroptosis. Our team of experts has many years of experience in the field and employs a variety of experimental and computational techniques to help clients deeply investigate and elucidate the mechanisms and pathways that regulate ferroptosis in stroke.

In Vitro Analysis of Ferroptosis Pathways in Stroke

We provide in vitro models of stroke to assess the activation of ferroptosis pathways and evaluate the effects on neuronal viability and function. We employ a range of techniques to measure key markers of ferroptosis including lipid peroxidation, iron accumulation, and expression levels of ferroptosis-related genes such as GPX4, ACSL4, and SLC7A11. In addition, we use advanced imaging techniques to study morphological changes associated with ferroptosis, such as plasma membrane alterations and mitochondrial dysfunction.

In Vivo Analysis of Ferroptosis Pathways in Stroke

In conjunction with in vitro studies, we use animal models of ischemic stroke for in vivo experiments. By inducing focal or global cerebral ischemia in animals, we can assess the progression of ferroptosis and its impact on neurological outcomes. In addition, through a combination of behavioral assessments, histological analyses, and molecular analyses, we help clients elucidate the neuronal damage, activation of ferroptosis pathways, and discover potential therapeutic targets to mitigate stroke-induced injury.

Downstream Pathways of Ferroptosis Pathways Potential Therapeutic Targets
Iron metabolic pathways FFerritin, transferrin, TFR1, metal ion transport protein 1 (DMT1)
Amino acid metabolic pathways GSH, GPX4
Lipid metabolic pathways LOX, ACSL4, Nrf2
Ferroptosis-related transcription pathways Tumor suppressor protein p53 (TP53), BAP1, HIF and YAP/YAP1

Stroke Drug Development Services Targeting Ferroptosis

Treatment of ischemic stroke by inhibiting ferroptosis has become a hot research topic. We can help clients develop stroke drugs targeting ferroptosis pathways.

  • Ferroptosis inhibitors
  • Iron homeostasis modulators
  • Lipid peroxidation pathway inhibitors
  • ROS generation inhibitors

With our expertise, state-of-the-art facilities, and collaborative approach, Ace Therapeutics provides in-depth research on the mechanism of ferroptosis and its role in stroke, helping clients find therapeutic targets for stroke. If you are interested in our services, please do not hesitate to contact us!

  1. Wei, Z., et al. (2022). New insights in ferroptosis: Potential therapeutic targets for the treatment of ischemic stroke. Frontiers in Pharmacology, 13, 1020918.
All of our services are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
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