Neurochemistry Service in Stroke Drug Discovery & Development

The mechanisms involved in stroke are complex and include neuronal excitotoxicity, energy metabolism imbalance, oxidative stress, inflammation, and apoptosis. In addition, ischemic stroke is also associated with metabolic disturbances such as lactate, creatine, N-acetyl aspartate, and choline. Real-time monitoring of these neurochemical changes in the brain after stroke allows for more accurate and sensitive pharmacokinetic and pharmacodynamic studies, which can help the development of stroke drugs.

Fig. 1. The scheme of the neurochemical processes in the brain after ischemic stroke leading to necrotic or apoptotic cell death and penumbra formation. (Uzdensky et al., 2020)

Our Neurochemistry Assay Service

At Ace Therapeutics, we are at the forefront of stroke research. We offer a variety of neurochemical bioanalytical tools to analyze neurological changes in the brain after stroke. In addition, we can develop customized study designs based on your specific needs for sample preparation, quantification, and analysis.

• Liquid chromatography-mass spectrometry (LC-MS)
• HPLC-electrochemical detection
• HPLC-fluorescence detection
• Enzyme-linked immunosorbent assay (ELISA)
• Radioimmunoassay (RIA)

Neurochemistry Analytes

Ace Therapeutics can characterize a wide range of neurochemical components to support interrogation of neurotransmitter kinetics, metabolic pathways, and biomarker profiles in animal models of stroke.

• Neurotransmitters: Dopamine, serotonin, acetylcholine, glutamate, GABA (gamma-aminobutyric acid), norepinephrine
• Neurotransmitter metabolite assays: Homovanillic acid (HVA), 5-hydroxyindoleacetic acid (5-HIAA)
• Cyclic adenosine monophosphate/cyclic guanosine
• Monoamines (including NE, DA, and 5HT)
• Polyamines (including putrescine and spermine)
• Glucose and lactate
• Glucocorticoids
• Kynurenine
• Tryptophan
• Histamine
• Custom analytes

Sample Type

• Brain tissue homogenate
• Cerebrospinal fluid (CSF)
• Neuronal cell culture
• Plasma/serum
• Urine and saliva

Ace Therapeutics provides comprehensive neurochemical assays to help deepen our understanding of the mechanisms of stroke and evaluate the neurochemical effects of stroke drug candidates. Our dedicated team provides customized solutions for your stroke drug development program based on your specific requirements. If you are interested in our services, please do not hesitate to contact us!

1. Uzdensky, A. B. (2020). Regulation of apoptosis in the ischemic penumbra in the first day post-stroke. Neural regeneration research, 15(2), 253-254.